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Mobilization of Pluripotent Multilineage-Differentiating Stress-Enduring Cells in Ischemic Stroke

Emiko Hori1, Yumiko Hayakawa2, Tomohide Hayashi2

  • 1Department of Neurosurgery, Graduate School of Medicine and Pharmacological Sciences, University of Toyama, Toyama, Japan; Department of Neurosurgery, Saiseikai Toyama Hospital, Toyama, Japan.

Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association
|March 30, 2016
PubMed
Abstract

Insights

Multilineage-differentiating stress-enduring (Muse) cells mobilize from bone marrow to blood after ischemic stroke. Smoking and alcohol intake influence Muse cell levels, suggesting potential therapeutic targets for stroke recovery.

Area of Science:

  • Stem Cell Biology
  • Neurology
  • Regenerative Medicine

Background:

  • Multilineage-differentiating stress-enduring (Muse) cells are pluripotent stem cells with regenerative potential.
  • Mobilization of endogenous stem cells is a key factor in tissue repair following injury.

Purpose of the Study:

  • To investigate the mobilization of Muse cells from bone marrow into peripheral blood in patients with ischemic stroke.
  • To determine the factors influencing the temporal profile of circulating Muse cells.

Main Methods:

  • Prospective study of 29 ischemic stroke patients, with peripheral blood sampling at admission, day 7, and day 30.
  • Quantification of Muse cells using fluorescence-activated cell sorting (stage-specific embryonic antigen-3 positivity).
  • Immunohistochemistry to assess Muse cell distribution in bone marrow from autopsy cases.

Main Results:

  • Muse cell numbers significantly increased in peripheral blood within 24 hours post-stroke onset compared to controls.
  • Baseline Muse cell levels and temporal profiles varied significantly among patients.
  • Smoking and alcohol intake were identified as significant factors affecting the increase in circulating Muse cells (OR=0.0027 for smoking, OR=1688 for alcohol).
  • Muse cells constituted approximately 0.20% ± 0.17% of bone marrow cells.

Conclusions:

  • Pluripotent Muse cells are mobilized from bone marrow into peripheral blood during the acute phase of ischemic stroke.
  • Smoking and alcohol consumption significantly impact the dynamics of Muse cell mobilization.
  • Therapeutic strategies involving endogenous Muse cell enhancement or exogenous Muse cell administration hold promise for improving functional outcomes in ischemic stroke patients.
Keywords:
Ischemic strokeMuse cellbone marrowmobilizationpluripotent stem cell

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