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Updated: Mar 23, 2026
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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
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Postprandial lipids accelerate and redirect nitric oxide consumption in plasma
Kurt Vrancken1, Hobe J Schroeder2, Lawrence D Longo2
1Division of Neonatology, Department of Pediatrics, Loma Linda University School of Medicine, USA.
Nitric Oxide : Biology and Chemistry
|March 30, 2016
Summary
High-fat meals increase plasma lipids, accelerating nitric oxide (NO) metabolism. This shift in NO consumption alters byproduct formation, impacting NO bioactivity.
Area of Science:
- Biochemistry
- Physiology
Background:
- Nitric oxide (NO) and O2 exhibit higher solubility in lipids than aqueous solutions.
- Elevated concentrations in plasma lipids can accelerate NO autoxidation, potentially affecting NO bioactivity.
Purpose of the Study:
- To investigate if increased plasma lipids post-high-fat meal accelerate NO metabolism.
- To determine if NO byproduct formation is altered by postprandial hyperlipidemia.
Main Methods:
- Comparison of NO consumption in postprandial vs. fasting plasma.
- Lipid removal experiments and addition of hydrophobic micelles.
- Analysis of NO consumption products (nitrite and SNO).
Main Results:
- Postprandial plasma showed increased NO consumption, correlated with triglyceride levels.
- Accelerated NO consumption was lipid-dependent and not due to hemoglobin or ceruloplasmin.
- Postprandial plasma yielded more nitrite and less SNO compared to fasting plasma.
Conclusions:
- Postprandial triglyceride-rich lipoproteins significantly influence NO metabolism in plasma.
- Lipid-accelerated NO autoxidation is approximately 48-fold.
- Dietary fat intake impacts NO bioavailability through altered plasma metabolism.
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