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Development of simple sequence repeat (SSR) markers for discrimination among isolates of Fusarium proliferatum
I Moncrief1, C Garzon1, S Marek1
1Oklahoma State University, Stillwater, OK, United States.
Journal of Microbiological Methods
|March 30, 2016
Summary
Fusarium proliferatum, a plant pathogen causing mycotoxin contamination, was studied using simple sequence repeat (SSR) markers. These SSR markers effectively characterized F. proliferatum isolates from various hosts and locations, aiding in understanding its spread.
Area of Science:
- Plant pathology
- Mycology
- Molecular genetics
Background:
- Fusarium proliferatum is a widespread plant pathogen known to produce harmful mycotoxins.
- An outbreak of onion salmon blotch caused by F. proliferatum in Israel highlighted the need to understand its source and distribution.
- Previous methods for characterizing F. proliferatum isolates were insufficient for diverse hosts and locations.
Purpose of the Study:
- To develop and characterize simple sequence repeat (SSR) markers for Fusarium proliferatum.
- To assess the utility of these SSR markers for differentiating F. proliferatum isolates from various hosts and geographic origins.
- To evaluate the transferability of developed SSR markers to other Fusarium species.
Main Methods:
- Inter-simple sequence repeats (ISSR) were employed to identify repetitive DNA motifs.
- Seventeen SSR loci were developed from ISSR data.
- Six polymorphic SSR markers were selected and tested on isolates from diverse hosts (cucumber, onion, garlic, maize, asparagus) and geographic regions (Israel, Germany, Austria, North America).
- SSR loci amplification was tested across seven related Fusarium species.
Main Results:
- Six SSR markers were polymorphic and consistently amplified from F. proliferatum isolates.
- These markers revealed 5 to 12 copies of di-, tri-, and pentanucleotide motifs, with 6 to 9 alleles per marker.
- The developed SSR markers successfully differentiated F. proliferatum isolates from different hosts and locations.
- Sixteen SSR loci showed amplification in at least one of the seven tested Fusarium species, indicating broad applicability.
Conclusions:
- Developed SSR markers are effective tools for characterizing F. proliferatum populations.
- These SSR markers are transferable and useful for studying genetic diversity across different Fusarium species.
- The findings contribute to understanding the epidemiology and genetic makeup of F. proliferatum.
