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Updated: Mar 23, 2026
Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
Published on: May 2, 2025
Metabolism-related enzyme alterations identified by proteomic analysis in human renal cell carcinoma
Zejun Lu1, Yuqin Yao2, Qi Song3
1Department of Radiation Oncology, Naval General Hospital of People's Liberation Army, Beijing, Chengdu, People's Republic of China.
Abstract:
The renal cell carcinoma (RCC) is one of the most common types of kidney neoplasia in Western countries; it is relatively resistant to conventional chemotherapy and radiotherapy. Metabolic disorders have a profound effect on the degree of malignancy and treatment resistance of the tumor. However, the molecular characteristics related to impaired metabolism leading to the initiation of RCC are still not very clear. In this study, two-dimensional electrophoresis (2-DE) and mass spectra (MS) technologies were utilized to identify the proteins involved in energy metabolism of RCC. A total of 73 proteins that were differentially expressed in conventional RCC, in comparison with the corresponding normal kidney tissues, were identified. Bioinformatics analysis has shown that these proteins are involved in glycolysis, urea cycle, and the metabolic pathways of pyruvate, propanoate, and arginine/proline. In addition, some were also involved in the signaling network of p53 and FAS. These results provide some clues for new therapeutic targets and treatment strategies of RCC.
Insights
Researchers identified 73 differentially expressed proteins in renal cell carcinoma (RCC) linked to energy metabolism. These findings offer potential new therapeutic targets for this chemotherapy-resistant kidney cancer.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Renal cell carcinoma (RCC) is a prevalent kidney neoplasia in Western nations.
- RCC exhibits resistance to conventional chemotherapy and radiotherapy.
- The molecular basis of metabolic dysregulation in RCC initiation remains unclear.
Purpose of the Study:
- To identify proteins involved in energy metabolism in renal cell carcinoma.
- To understand the molecular characteristics of impaired metabolism in RCC.
Main Methods:
- Utilized two-dimensional electrophoresis (2-DE) and mass spectrometry (MS).
- Compared protein expression in conventional RCC tissues versus normal kidney tissues.
Main Results:
- Identified 73 differentially expressed proteins in RCC.
- Proteins are involved in glycolysis, urea cycle, pyruvate, propanoate, and arginine/proline metabolic pathways.
- Some identified proteins are implicated in p53 and FAS signaling networks.
Conclusions:
- The study provides insights into metabolic alterations in RCC.
- Identified proteins may serve as novel therapeutic targets for RCC treatment.
- Findings suggest new strategies for managing treatment-resistant kidney cancer.
