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Updated: Mar 23, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Suspended sludge and biofilm shaped different anammox communities in two pilot-scale one-stage anammox reactors
Bingyu Zheng1, Liang Zhang2, Jianhua Guo1
1Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing, China.
Abstract:
The abundance and diversity of anammox bacteria was investigated in two pilot-scale integrated fixed-film activated sludge (IFAS) reactors treating high ammonium wastewater. Reactor A was inoculated with nitrifying sludge, while Reactor B was inoculated with suspended anammox sludge with the dominant anammox bacteria of Candidatus 'Kuenenia'. After 180days' operation, the predominate anammox bacteria was Candidatus 'Brocadia' (65%) in the biofilm, while Candidatus 'Kuenenia' (86%) outcompeted with other anammox bacteria in suspended sludge in Reactor A. Candidatus 'Kuenenia' were dominated in suspended sludge through the entire experiment in Reactor B. In contrast, the predominated species shifted from Candidatus 'Kuenenia' (89%) into Candidatus 'Brocadia' (66%) in the biofilm of Reactor B. This study indicated that Candidatus 'Brocadia' preferred to grow in the biofilm, while Candidatus 'Kuenenia' would dominant over other anammox bacteria in the suspended sludge. Further studies are required to identify the internal factors affecting the distribution of anammox bacteria.
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