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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Studying the Cytoskeleton01:17

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The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Related Experiment Video

Updated: Mar 23, 2026

Peering at Brain Polysomes with Atomic Force Microscopy
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Peering at Brain Polysomes with Atomic Force Microscopy

Published on: March 16, 2016

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Peering at Brain Polysomes with Atomic Force Microscopy.

Lorenzo Lunelli1, Paola Bernabò2, Alice Bolner2

  • 1Laboratory of Biomolecular Sequence and Structure Analysis for Health, Fondazione Bruno Kessler.

Journal of Visualized Experiments : Jove
|March 30, 2016
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method using atomic force microscopy (AFM) to visualize polysome organization. This technique allows for 3D reconstruction of these crucial cellular structures under near-physiological conditions.

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Peering at Brain Polysomes with Atomic Force Microscopy
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Area of Science:

  • Molecular and Cellular Biology
  • Biophysics
  • Neuroscience

Background:

  • Polysomes (polyribosomes) are large, complex cytoplasmic machines essential for protein synthesis.
  • While ribosomes are well-studied, polysome organization and associated translational control mechanisms remain poorly understood.
  • Elucidating polysome structure is key to uncovering novel regulatory pathways in gene expression.

Purpose of the Study:

  • To establish a detailed protocol for purifying polysomes from mouse brain.
  • To enable high-resolution 3D imaging and analysis of polysome organization using Atomic Force Microscopy (AFM).
  • To provide a complementary technique to cryo-electron microscopy (cryo-EM) for studying polysomes.

Main Methods:

  • Accurate purification of polysomes from mouse brain tissue.
  • Deposition of purified polysomes onto mica substrates for imaging.
  • Atomic Force Microscopy (AFM) for nanoscale 3D imaging in air and solution.
  • Reconstruction of polysomes as three-dimensional objects.

Main Results:

  • A reliable protocol for polysome purification and deposition was successfully established.
  • AFM enabled high-resolution imaging of polysomes under near-physiological conditions.
  • The method allows for the 3D reconstruction and detailed analysis of polysome organization.

Conclusions:

  • The developed AFM protocol offers a powerful tool for investigating polysome organization.
  • This technique complements existing methods like cryo-EM for comprehensive polysome analysis.
  • Further studies using this method can reveal novel insights into translational control mechanisms.