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Quantitative Probing of Cu(2+) Ions Naturally Present in Single Living Cells
Junho Lee1, Hwa-Rim Lee2, Jaeyeon Pyo1
1X-Ray Imaging Center, Department of Materials Science and Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea.
Abstract:
Quantitative probing of Cu(2+) ions naturally present in single living cells is realized by developing a quantum-dot-embedded nanowire-waveguide probe. The intracellular Cu(2+) ion concentration is quantified by direct monitoring of photoluminescence quenching during the insertion of the nanowire in a living neuron. The measured intracellular Cu(2+) ion concentration is 3.34 ± 1.04 × 10(-6) m (mean ± s.e.m.) in single hippocampal neurons.
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