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Sensitive, direct colorimetric assay for copper in serum.

A Abe¹, S Yamashita, A Noma

¹Department of Laboratory Medicine, Gifu University School of Medicine, Japan.

Clinical Chemistry

|April 1, 1989

Summary

A new method accurately measures serum copper using a specific color reagent and spectrophotometry. This sensitive assay offers reliable results for clinical diagnostics and research applications.

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Area of Science:

Clinical Chemistry
Analytical Chemistry

Background:

Accurate determination of serum copper is crucial for diagnosing and monitoring various health conditions.
Existing methods may have limitations in sensitivity or specificity.

Purpose of the Study:

To develop and validate a sensitive and reliable colorimetric method for serum copper determination.
To assess the performance characteristics of the new assay, including linearity, detection limits, and precision.

Main Methods:

A novel color reagent, 4-(3,5-dibromo-2-pyridylazo)-N-ethyl-N-sulfopropylaniline, was synthesized and utilized.
Serum samples were incubated with the reagent, and the resulting chelate complex absorbance was measured at 580 nm.
The method's performance was evaluated against atomic absorption spectrophotometry.

Main Results:

The method demonstrated linearity with copper concentration up to 5 mg/L, with a low detection limit of 0.1 mg/L.
High precision was observed with within-run coefficients of variation (CVs) of 1.6% and 3.3%, and a between-run CV of 2.8%.
Excellent correlation (r = 0.977) was found with atomic absorption spectrophotometry, indicating good accuracy.

Conclusions:

The developed colorimetric method provides a sensitive, precise, and accurate means for serum copper determination.
This assay is suitable for routine clinical laboratory use and research, with minimal interference from common metal ions.

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