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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Mar 23, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Overcoming obstacles in localization microscopy

Ralf Jungmann¹

¹Department of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany, and the Max Planck Institute of Biochemistry, Martinsried, Germany.

|March 31, 2016

Summary

No abstract available in PubMed .

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Last Updated: Mar 23, 2026

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