Jove
Visualize
Contact Us

Related Experiment Videos

Polymorphonuclear neutrophils function in splenectomized patients.

H Wysocki1, B Wierusz-Wysocka, H Karoń

  • 1Institute of Internal Medicine, Academy of Medicine, Poznań, Poland.

Clinical and Experimental Immunology
|March 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of optimum classification measures used to define textbook outcome among patients undergoing curative-intent resection of gastric cancer.

BMC cancer·2023
Same author

Nomogram-Based Prognostic Evaluation of Gastric Cancer Patients with Low Counts of Examined Lymph Nodes Outperforms the Predictive Ability of the 7<sup>th</sup> and 8<sup>th</sup> Editions of the American Joint Committee on Cancer Staging System.

Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract·2022
Same author

Associations between heart rate asymmetry expression and asymmetric detrended fluctuation analysis results.

Medical & biological engineering & computing·2022
Same author

Testing heart rate asymmetry in long, nonstationary 24 hour RR-interval time series.

Physiological measurement·2019
Same author

The relationship between sudomotor function and skin microvascular reactivity in individuals with type 1 diabetes of long duration.

Microvascular research·2018
Same author

Association between serum adiponectin and HbA1c is independent of the severity of clinical presentation in young adults with new onset type 1 diabetes.

Journal of endocrinological investigation·2016
Same journal

Could we discuss the molecular signature of immune dysregulation?

Clinical and experimental immunology·2026
Same journal

The many faces of cytokine storm syndrome: immunopathogenic mechanisms and clinical implications for a better patient management.

Clinical and experimental immunology·2026
Same journal

PD-1 Engagement and Circulating Th17 Effector Activity During Secukinumab Treatment in Plaque Psoriasis.

Clinical and experimental immunology·2026
Same journal

Henoch-Schoenlein purpura (HSP) like lesions in IL12RB1 and IL12B defects - A multi-centric experience from India.

Clinical and experimental immunology·2026
Same journal

Case Series on the Efficacy of Daratumumab in Treating Patients with Anti-Interferon-Gamma Autoantibodies.

Clinical and experimental immunology·2026
Same journal

Deleterious germline CARD11 gain-of-function variants alter human B-cell and CD4+ T-cell differentiation and function.

Clinical and experimental immunology·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Splenectomy impairs key polymorphonuclear neutrophil (PMN) functions, including chemotaxis and bacterial killing, despite normal initial responses. These immune cell changes may affect infection risk in patients post-splenectomy.

Area of Science:

  • Immunology
  • Hematology

Background:

  • Splenectomy, the surgical removal of the spleen, can impact immune function.
  • Polymorphonuclear neutrophils (PMN) are critical immune cells involved in fighting bacterial infections.

Purpose of the Study:

  • To evaluate the functional status of PMN in patients who have undergone splenectomy due to splenic rupture.
  • To identify specific PMN functions that are altered after splenectomy.

Main Methods:

  • Assessed random migration, adherence, phagocytosis, chemotaxis, and bactericidal capacity of PMN.
  • Measured unstimulated and stimulated oxygen (O2) and hydrogen peroxide (H2O2) production.
  • Quantified intracellular myeloperoxidase activity and Fc IgG receptor expression on PMN.

Main Results:

Related Experiment Videos

  • PMN showed normal random migration and adherence but impaired chemotaxis, aggregation, and stimulated O2/H2O2 production.
  • Phagocytosis of staphylococci was enhanced, while bactericidal capacity was reduced.
  • Decreased intracellular myeloperoxidase activity and Fc IgG receptor expression were observed.
  • Patient plasma increased neutrophil adherence, suggesting plasma-mediated effects.

Conclusions:

  • Splenectomy significantly impairs crucial PMN functions essential for effective bacterial clearance.
  • Altered PMN function and reduced Fc IgG receptor expression may contribute to increased infection susceptibility in asplenic individuals.