Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.4K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.4K
pre-mRNA Processing02:01

pre-mRNA Processing

58.1K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
58.1K
Pre-mRNA Processing02:01

Pre-mRNA Processing

29.8K
29.8K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

17.0K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
17.0K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

3.6K
3.6K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

7.6K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FGFR1 drives metabolic adaptation associated with temozolomide resistance in glioblastoma.

Cancer letters·2026
Same author

Circadian regulation of homologous recombination by cryptochrome1-mediated dampening of DNA end resection.

Nature communications·2025
Same author

DNA topoisomerase II promotes N<sup>6</sup>-adenosine mRNA methylation.

Cell reports·2025
Same author

SARS-CoV-2 E protein interacts with BRD2 and BRD4 SEED domains and alters transcription in a different way than BET inhibition.

Cellular and molecular life sciences : CMLS·2024
Same author

Serpine1 mRNA confers mesenchymal characteristics to the cell and promotes CD8+ T cells exclusion from colon adenocarcinomas.

Cell death discovery·2024
Same author

SWI/SNF-dependent genes are defined by their chromatin landscape.

Cell reports·2024

Related Experiment Video

Updated: Mar 23, 2026

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

7.9K

Chromatin structure and pre-mRNA processing work together.

Silvia Jimeno-González1, José C Reyes1

  • 1a Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Consejo Superior de Investigaciones Científicas (CSIC) , Seville , Spain.

Transcription
|March 31, 2016
PubMed
Summary

Transcription pauses by RNA polymerase II (RNAPII) in chromatin may coordinate gene expression with pre-mRNA processing. Histone modifications further regulate this crucial step in mature messenger RNA synthesis.

Keywords:
mRNA cappingnucleosomepromoter proximal pausingsplicingtranscription termination

More Related Videos

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.5K
Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
09:16

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

13.2K

Related Experiment Videos

Last Updated: Mar 23, 2026

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

7.9K
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.5K
Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
09:16

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

13.2K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Expression

Background:

  • Chromatin structure, specifically nucleosomes, naturally impedes RNA polymerase II (RNAPII) during transcription elongation.
  • RNAPII pausing within gene bodies is a common occurrence.
  • Coordinating transcription with pre-mRNA processing is essential for accurate gene expression.

Purpose of the Study:

  • To explore the regulatory role of RNAPII pausing in coordinating transcription elongation with pre-mRNA processing.
  • To highlight the influence of chromatin context and histone modifications on mRNA synthesis.

Main Methods:

  • Literature review and synthesis of current research findings.
  • Discussion of experimental evidence linking transcription pausing to mRNA processing.
  • Analysis of the role of histone post-translational modifications.

Main Results:

  • Evidence suggests that RNAPII pausing is not merely a roadblock but a regulatory mechanism.
  • Pausing facilitates the timely recruitment of factors necessary for pre-mRNA processing.
  • Histone modifications dynamically regulate the interaction between chromatin, RNAPII, and processing factors.

Conclusions:

  • The chromatin context, through nucleosome positioning and histone modifications, plays a critical regulatory role in gene expression.
  • RNAPII pausing is an integral part of the mechanism linking transcription elongation to pre-mRNA processing.
  • Understanding these chromatin-mediated regulatory events is key to comprehending mature mRNA synthesis.