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Multidrug Efflux Systems in Helicobacter cinaedi.
Yuji Morita1, Junko Tomida2, Yoshiaki Kawamura3
1Department of Microbiology, School of Pharmacy, Aichi Gakuin University/ 1-100 Kusumoto, Chikusa, Nagoya, Aichi 464-8650, Japan. yujmor@dpc.agu.ac.jp.
Antibiotics (Basel, Switzerland)
|April 1, 2016
Summary
Helicobacter cinaedi infections are difficult to treat due to slow growth and emerging antimicrobial resistance. This study compares its multidrug efflux systems to other bacteria, aiding future treatment strategies.
Area of Science:
- Microbiology
- Genetics
- Infectious Diseases
Background:
- Helicobacter cinaedi causes serious infections, particularly in immunocompromised individuals.
- Slow growth and poor subculture characteristics hinder H. cinaedi analysis and resistance studies.
- Antimicrobial resistance is a growing concern for H. cinaedi infections, necessitating understanding of resistance mechanisms.
Purpose of the Study:
- To investigate the multidrug efflux systems of Helicobacter cinaedi.
- To compare H. cinaedi efflux systems with those found in other bacterial species.
- To contribute to understanding antimicrobial resistance in H. cinaedi.
Main Methods:
- Whole genome sequencing of H. cinaedi strain PAGU 611.
- Analysis of identified multidrug efflux systems.
- Comparative analysis of efflux system organization and properties.
Main Results:
- H. cinaedi strain PAGU 611 possesses multidrug efflux systems.
- The strain exhibits intrinsic antimicrobial resistance mechanisms.
- Mutations in gyrA and 23S rRNA gene confer resistance to fluoroquinolones and macrolides.
Conclusions:
- Understanding H. cinaedi's efflux systems is crucial for combating antimicrobial resistance.
- Comparative analysis provides insights into H. cinaedi's resistance strategies.
- Further development of genetic tools for H. cinaedi is needed.
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