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Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro
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Gene Electrotransfer: A Mechanistic Perspective.

Christelle Rosazza, Sasa Haberl Meglic, Andreas Zumbusch

  • 1Institute of Pharmacology and Structural Biology (IPBS), CNRS UMR5089, 205 route de Narbonne, 31077 Toulouse, France. Marie-Pierre.Rols@ipbs.fr.

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Gene electrotransfer uses electric fields to enhance DNA delivery for medical applications like cancer gene therapy and DNA vaccination. This review details the process, from membrane permeabilization to intracellular DNA trafficking, for improved understanding.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biophysics

Background:

  • Gene electrotransfer is a promising DNA delivery method for gene therapy and vaccination.
  • Electroporation transiently alters cell membrane permeability using electric fields.
  • Despite extensive study, DNA entry and intracellular transport mechanisms remain unclear.

Purpose of the Study:

  • To provide a comprehensive review of gene electrotransfer knowledge accumulated over three decades.
  • To outline membrane electropermeabilization as a key DNA delivery process.
  • To detail DNA internalization and intracellular trafficking mechanisms.

Main Methods:

  • Review of existing literature on gene electrotransfer.
  • Analysis of membrane electropermeabilization principles.
  • Step-by-step description of DNA internalization and trafficking.
  • Discussion of in vivo testing and methodologies.

Main Results:

  • Gene electrotransfer offers significant medical applications, including cancer gene therapy and DNA vaccination.
  • Membrane electropermeabilization is crucial for DNA and molecule delivery.
  • Current understanding of DNA internalization and intracellular trafficking is evolving.
  • In vivo applications and methodologies are key considerations.

Conclusions:

  • A comprehensive understanding of gene electrotransfer mechanisms is essential for optimizing its medical applications.
  • Further research into DNA internalization and intracellular trafficking will enhance gene delivery efficiency.
  • Standardized in vivo methodologies are needed for reliable gene electrotransfer applications.