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Related Concept Videos

Methods of Sterilization II: Chemical Methods01:30

Methods of Sterilization II: Chemical Methods

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In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
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Radical Autoxidation01:20

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The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
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Methods of Sterilization I: Physical Methods01:29

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As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
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Oxidation of Alcohols02:37

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In this lesson, the oxidation of alcohols is discussed in depth. The various reagents used for oxidation of primary and secondary alcohols are detailed, and their mechanism of action is provided.
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Chemical Agents for Microbial Control01:27

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Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
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Cleaning, Sterilization, and Disinfection01:30

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Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
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Related Experiment Video

Updated: Mar 23, 2026

Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte
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Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte

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Advanced oxidation process sanitization of eggshell surfaces.

Steven M Gottselig1, Sadie L Dunn-Horrocks1, Kristy S Woodring1

  • 1Department of Poultry Science, Texas A&M University, College Station.

Poultry Science
|April 1, 2016
PubMed
Summary
This summary is machine-generated.

The hydrogen peroxide and ultraviolet light (H2O2/UV) process effectively sanitizes eggs by reducing surface bacteria. Optimized conditions include two cycles of 5-second UV exposure, significantly lowering Salmonella contamination on eggshells.

Keywords:
Salmonellaadvanced oxidation processeggshellhydrogen peroxideultraviolet light

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Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods
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Area of Science:

  • Food Science
  • Microbiology
  • Poultry Production

Background:

  • Egg microbial quality is crucial for hatchery biosecurity and food safety.
  • Effective interventions are needed to reduce egg surface contamination.
  • Hydrogen peroxide and ultraviolet light (H2O2/UV) offer a promising alternative to traditional egg sanitizers.

Purpose of the Study:

  • To optimize treatment conditions for the H2O2/UV advanced oxidation process for egg sanitation.
  • To identify practical operational parameters for applying H2O2/UV technology in commercial settings.
  • To evaluate the efficacy of H2O2/UV in reducing microbial load and Salmonella on egg surfaces.

Main Methods:

  • Evaluated the impact of application cycles, UV intensity, UV exposure duration, and egg rotation.
  • Assessed total aerobic bacteria reduction on naturally contaminated eggs.
  • Determined Salmonella reduction on experimentally contaminated eggs using optimized H2O2/UV conditions.

Main Results:

  • Optimized H2O2/UV sanitation involved 2 cycles of 5-second exposure to 14 mW cm⁻² UV light.
  • Egg rotation was found to be unnecessary for effective microbial reduction.
  • Optimized conditions reduced Salmonella by over 5 log10 cfu/egg on contaminated eggshells.

Conclusions:

  • The H2O2/UV advanced oxidation process is a viable technology for practical egg sanitation.
  • Optimized H2O2/UV treatment significantly reduces Salmonella contamination on egg surfaces.
  • This method enhances poultry production safety and food microbial safety.