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Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements
Shona A Mookerjee1,2, David G Nicholls2, Martin D Brand1,2
1Touro University California College of Pharmacy, 1310 Club Drive, Vallejo, California, 94592, United States of America.
Plos One
|April 1, 2016
Summary
This study defines maximum glycolytic capacity and differentiates it from glycolytic rate. Extracellular flux analysis provides a quantitative method to measure these crucial cellular energy parameters.
Area of Science:
- Cellular energetics
- Metabolic analysis
Background:
- Glycolytic rate and capacity are key indicators of cell status.
- Understanding these parameters is vital for studying cell differentiation, pathology, and drug responses.
Purpose of the Study:
- To experimentally define maximum glycolytic capacity.
- To differentiate maximum glycolytic capacity from basal glycolytic rate.
- To provide a protocol for measuring both using extracellular flux analysis.
Main Methods:
- Utilized extracellular flux analysis.
- Developed a protocol for measuring basal glycolytic rate.
- Developed a protocol for measuring maximum glycolytic capacity.
Main Results:
- Successfully defined and differentiated maximum glycolytic capacity from glycolytic rate.
- Demonstrated the utility of extracellular flux analysis for quantitative assessment of cellular energetics.
- Provided a reproducible protocol for measuring glycolytic parameters.
Conclusions:
- Extracellular flux analysis is a powerful tool for quantitatively assessing cellular energetics in adherent cells.
- Accurate measurement of glycolytic rate and capacity offers critical insights into cell behavior and responses.
