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Regional localization and molecular characterization of a DNA sequence on the long arm of chromosome 22
E Göttert1, R Metzdorf, U Färber
1Institut für Humangenetik, Universitätsklinik, Homburg/Saar, Federal Republic of Germany.
Human Genetics
|March 1, 1989
Abstract:
A human genomic DNA fragment, p22hom13 (D22S16), was isolated from a chromosome 22-specific library. After elimination of repetitive sequences, a single copy BamHI-EcoRI fragment was subcloned into pTZ18. By using mouse/human somatic cell hybrids and in situ hybridization, the new DNA probe was mapped to chromosome 22q13-qter. Its application in the analysis of the distal part of chromosome 22 and its diagnostic use in translocations are discussed.