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Updated: Mar 23, 2026

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
Published on: March 31, 2011
Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro
Yukiteru Ouji1, Masahide Yoshikawa2
1Department of Pathogen, Infection and Immunity, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara, 634-8521, Japan. oujix@naramed-u.ac.jp.
Abstract:
CD49f+ CD34+ cells, a population rich in skin epithelial stem cells (EpSCs), were obtained from adult mouse skin and cultured with Wnt-3a for 10 days. On day 10, CD49f+ CD34+ cells were sorted and subjected to a second 10-day culture with Wnt-3a. The same procedures were repeated until fifteenth 10-day culture. CD49f+ CD34+ cells obtained from each 10-day culture retained the same EpSC-characteristics as seen in the original EpSCs from adult mouse skin. Here, wedescribe the culture protocol using Wnt-3a for successful maintenance of EpSCs.
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