Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbiota of the Large Intestine01:27

Microbiota of the Large Intestine

2
The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...
2

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diurnal Variation of Islet Autoantibody Titers in Established Type 1 Diabetes Suggests Restricted-Time Sampling Improves Aab Measurement and Detection.

Research square·2026
Same author

IL17-deficient NOD mice are protected from autoimmune diabetes due to decreased antigen presentation and T cell activation.

Frontiers in immunology·2026
Same author

CXCR3 expression on antigen-experienced B cells is systemically dysregulated in type 1 diabetes.

Diabetologia·2025
Same author

<i>Pdgfrα</i> deficiency in islet β-cells up-regulates apoptosis of beta-cells and disturbs glucose metabolism in B6 mice.

Frontiers in endocrinology·2025
Same author

Is the NOD mouse a good model for type 1 diabetes?

Diabetologia·2025
Same author

Alpha cells transdifferentiate into delta cells during the progression of autoimmunity in nondiabetic NOD mice.

American journal of physiology. Endocrinology and metabolism·2025

Related Experiment Video

Updated: Mar 23, 2026

Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method
05:28

Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method

Published on: May 2, 2022

2.6K

The Gut Microbiome in the NOD Mouse.

Jian Peng1, Youjia Hu1, F Susan Wong2

  • 1Department of Endocrinology and Metabolism, School of Medicine, Yale University, 208020, New Haven, CT, 06520-8020, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 2, 2016
PubMed
Summary

The gut microbiome influences type 1 diabetes in nonobese diabetic (NOD) mice. This protocol details gut bacteria analysis, including DNA isolation, sequencing, and data analysis for NOD mouse studies.

Keywords:
16S sequencingGut microbiomeNOD mice

More Related Videos

A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System
07:32

A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System

Published on: August 30, 2019

9.4K
Establishment and Maintenance of Gnotobiotic American Cockroaches Periplaneta americana
06:09

Establishment and Maintenance of Gnotobiotic American Cockroaches Periplaneta americana

Published on: May 28, 2021

5.7K

Related Experiment Videos

Last Updated: Mar 23, 2026

Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method
05:28

Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method

Published on: May 2, 2022

2.6K
A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System
07:32

A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System

Published on: August 30, 2019

9.4K
Establishment and Maintenance of Gnotobiotic American Cockroaches Periplaneta americana
06:09

Establishment and Maintenance of Gnotobiotic American Cockroaches Periplaneta americana

Published on: May 28, 2021

5.7K

Area of Science:

  • Microbiology
  • Immunology
  • Genetics

Background:

  • The human gut microbiome comprises microorganisms crucial for health and disease.
  • Gut microbiota alterations impact health, with increasing research linking composition to disease phenotypes.
  • The nonobese diabetic (NOD) mouse model is vital for studying spontaneous type 1 diabetes.

Purpose of the Study:

  • To present a standard protocol for gut microbiome analysis in NOD mice.
  • To outline methods for investigating the role of gut bacteria in type 1 diabetes development.
  • To provide a reproducible framework for microbiome research in diabetes models.

Main Methods:

  • Isolation of total DNA from gut bacteria in fecal or intestinal samples.
  • Bacterial DNA sequencing for microbial community profiling.
  • Basic data analysis for interpreting microbiome composition.

Main Results:

  • Established a routine protocol for gut microbiome analysis in NOD mice.
  • Enabled detailed investigation of gut bacteria's role in diabetes pathogenesis.
  • Provided a foundation for further research on microbiome-host interactions.

Conclusions:

  • The presented protocol facilitates comprehensive gut microbiome analysis in NOD mice.
  • Understanding the gut microbiome is critical for unraveling type 1 diabetes development.
  • This standardized approach supports reproducible research in diabetes and microbiome science.