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Updated: Mar 23, 2026
Investigating Intestinal Inflammation in DSS-induced Model of IBD
Published on: February 1, 2012
Fecal dysbiosis in miniature dachshunds with inflammatory colorectal polyps
Hirotaka Igarashi1, Koichi Ohno1, Ayako Horigome2
1Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Abstract:
Chronic gastrointestinal disease is associated with the alteration of gastrointestinal microbiota. Inflammatory colorectal polyps (ICRPs) are commonly observed in miniature dachshunds (MDs) in Japan and are characterized by multiple polyps that are restricted in the colorectal mucosa with severe neutrophil infiltration. This study was aimed to compare the fecal microbiota of ICRP-affected MDs with that of healthy MDs. High-throughput sequencing of amplicons derived from the V3-V4 region of the 16S rRNA gene was applied using the Illumina MiSeq system. Principal coordinates analysis revealed that fecal microbiota of ICRP-affected MDs was significantly altered compared with that of healthy MDs. Proportions of Fusobacteriaceae, Helicobacteraceae, Porphyromonadaceae, and Turicibacteraceae were significantly more abundant in ICRP-affected MDs, while those of Lachnospiraceae were significantly less abundant in ICRP-affected MDs compared with healthy MDs. These results suggest that the dysbiosis is associated with ICRPs and is a potential therapeutic target, though further investigations are needed.
Insights
Fecal microbiota is altered in miniature dachshunds with inflammatory colorectal polyps (ICRPs). Specific bacterial families like Fusobacteriaceae and Helicobacteraceae increase, while Lachnospiraceae decrease, suggesting dysbiosis as a therapeutic target.
Area of Science:
- Veterinary Medicine
- Microbiology
- Gastroenterology
Background:
- Chronic gastrointestinal diseases correlate with changes in gut microbiota.
- Inflammatory colorectal polyps (ICRPs) are prevalent in Japanese miniature dachshunds (MDs), marked by colorectal polyps and neutrophil infiltration.
Purpose of the Study:
- To compare the fecal microbiota composition between ICRP-affected MDs and healthy MDs.
- To identify specific microbial alterations associated with ICRPs in MDs.
Main Methods:
- High-throughput sequencing of 16S rRNA gene V3-V4 amplicons using Illumina MiSeq.
- Analysis of fecal microbiota using principal coordinates analysis (PCoA).
Main Results:
- Significant alterations in fecal microbiota were observed in ICRP-affected MDs compared to healthy controls.
- Increased abundance of Fusobacteriaceae, Helicobacteraceae, Porphyromonadaceae, and Turicibacteraceae in ICRP-affected MDs.
- Decreased abundance of Lachnospiraceae in ICRP-affected MDs.
Conclusions:
- The study suggests a link between gut dysbiosis and ICRPs in miniature dachshunds.
- Microbial alterations identified may represent potential therapeutic targets for ICRP management.
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