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Updated: Mar 23, 2026
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
Published on: June 7, 2016
Breast cancer upregulating genes in stromal cells by LINE-1 hypermethylation and micrometastatic detection
Charoenchai Puttipanyalears1,2, Nakarin Kitkumthorn3, Supranee Buranapraditkun4
1Inter-Department Program of BioMedical Sciences, Faculty of Graduate School, Chulalongkorn University, Bangkok, Thailand.
Unlabelled:
Changes in the methylation level of genes containing LINE-1 alter host gene regulation.
Aim:
This study demonstrates that paracrine signaling of breast cancer influences the epigenetic regulation of stromal cells.
Methods:
We proved in vitro and in vivo breast cancer promoted LINE-1 methylation exists exclusively in female stromal cells.
Results:
Genes containing LINE-1 of breast cancer stromal cells were upregulated. Furthermore, one of the genes, MUC-1, was demonstrated to have expression in plasma cells from the lymph nodes of patients with lymph node metastasis or micrometastasis.
Conclusion:
Breast cancer sends a paracrine signal to stroma cells causing LINE-1 epigenetic regulation. Moreover, the regulated genes in stroma cells are potential biomarkers for detecting breast cancer micrometastasis.
