Related Experiment Video

Updated: Mar 23, 2026

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.5K

Diallyl trisulfide induces osteosarcoma cell apoptosis through reactive oxygen species-mediated downregulation of the

Hongliang Wang1, Na Sun2, Xin Li1

  • 1Department of Orthopedics, Qilu Hospital, Shandong University, Ji'nan, Shandong 250012, P.R. China.

Oncology Reports
|April 2, 2016
PubMed

Insights

Diallyl trisulfide (DATS), a garlic compound, effectively inhibits osteosarcoma cell growth by inducing apoptosis. Its anticancer effects are mediated by reactive oxygen species (ROS) and the PI3K/Akt pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Diallyl trisulfide (DATS) is a natural organosulfur compound found in garlic with reported anticancer properties.
  • The specific mechanisms of DATS in human osteosarcoma cells remain underexplored.

Purpose of the Study:

  • To investigate the anticancer effects of DATS on human osteosarcoma cells.
  • To elucidate the molecular mechanisms underlying DATS-induced cell death.

Main Methods:

  • Cell viability assays in MG63 and MNNG/HOS osteosarcoma cell lines.
  • Flow cytometry to assess cell cycle phase distribution and mitochondrial membrane potential.
  • Measurement of reactive oxygen species (ROS) levels.
  • Western blotting to analyze key proteins in cell signaling pathways.
  • Inhibition studies using N-acetylcysteine (NAC) and LY294002.

Main Results:

  • DATS significantly reduced osteosarcoma cell viability in a dose- and time-dependent manner.
  • DATS induced G0/G1 cell cycle arrest, increased ROS production, and decreased mitochondrial membrane potential.
  • Apoptosis was induced via inhibition of the PI3K/Akt pathway, and this was found to be ROS-dependent.
  • N-acetylcysteine (NAC) treatment blocked DATS-induced ROS, PI3K/Akt inhibition, and apoptosis.

Conclusions:

  • DATS exhibits potent anticancer activity against human osteosarcoma cells.
  • The mechanism involves ROS-mediated downregulation of the PI3K/Akt pathway, leading to apoptosis.
  • DATS shows potential as a novel therapeutic agent for osteosarcoma.

More Related Videos

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro08:04

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

Published on: March 18, 2014

13.4K
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

12.0K

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
6.2K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
13.8K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
9.1K