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Updated: Aug 15, 2026
An Orthotopic Bladder Cancer Model for Gene Delivery Studies
Published on: December 1, 2013
Inhibiting cell migration and cell invasion by silencing the transcription factor ETS-1 in human bladder cancer
Li Liu1,2,3, Yuchen Liu1, Xintao Zhang1
1Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China.
Abstract:
As one of the members of the ETS gene family, the transcription factor v-ets avian erythroblastosis virus E26 oncogene homolog 1 (ETS-1) plays key role in the regulation of physiological processes in normal cells and tumors. In this study, we aimed to investigate the relationship between the transcription factor ETS-1 and malignant phenotypes of bladder cancer. We demonstrated that ETS-1 was up-regulated in human bladder cancer tissue compared to paired normal bladder tissue. In order to evaluate the functional role of ETS-1 in human bladder cancer, vectors expressing ETS-1 shRNA and ETS-1 protein were constructed in vitro and transfected into the human bladder cancer T24 and 5637 cells. Our results showed that the transcription factor ETS-1 could promote cell migration and cell invasion in human bladder cancer, without affecting cell proliferation and apoptosis. In conclusion, ETS-1 plays oncogenic roles through inducing cell migration and invasion in human bladder cancer, and it can be used as a therapeutic target for treating human bladder cancer.
Insights
The transcription factor ETS-1 is elevated in bladder cancer and promotes tumor cell migration and invasion. This suggests ETS-1 is an oncogenic driver and a potential therapeutic target for bladder cancer.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The ETS-1 (v-ets avian erythroblastosis virus E26 oncogene homolog 1) transcription factor is part of the ETS gene family.
- ETS-1 is involved in regulating physiological processes in both normal cells and tumors.
Purpose of the Study:
- To investigate the association between ETS-1 and the malignant phenotypes of human bladder cancer.
- To determine the functional role of ETS-1 in bladder cancer progression.
Main Methods:
- ETS-1 expression levels were compared between human bladder cancer tissues and adjacent normal tissues.
- In vitro studies involved transfecting bladder cancer cell lines (T24 and 5637) with vectors to modulate ETS-1 expression (shRNA for knockdown, protein for overexpression).
Main Results:
- ETS-1 was found to be significantly upregulated in human bladder cancer tissues compared to normal bladder tissues.
- Overexpression of ETS-1 enhanced cell migration and invasion in bladder cancer cell lines.
- ETS-1 modulation did not affect cell proliferation or apoptosis.
Conclusions:
- ETS-1 acts as an oncogene in human bladder cancer by promoting cell migration and invasion.
- ETS-1 represents a potential therapeutic target for the treatment of bladder cancer.
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