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Period2 downregulation inhibits glioma cell apoptosis by activating the MDM2-TP53 pathway

Niu Zhanfeng1,2, Wang Chengquan3, Xia Hechun1,2

  • 1Department of Neurosurgery, The General Hospital of Ningxia Medical University, Yinchuan, 750004, China.

Oncotarget
|April 2, 2016
PubMed

Insights

The Period2 (Per2) gene, a core circadian gene, acts as a tumor suppressor by enhancing DNA damage repair and apoptosis. Lowering Per2 expression reduces cell death in glioma, suggesting its role in cancer progression.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Chronobiology

Background:

  • The Period2 (Per2) gene is integral to the mammalian circadian clock.
  • Per2 is implicated in glioma development and response to radiotherapy.

Purpose of the Study:

  • To investigate the role of Per2 in response to X-ray-induced DNA damage.
  • To examine Per2's function in U343 glioma cells and a mouse cancer model.

Main Methods:

  • RNA interference (RNAi) to reduce Per2 expression.
  • X-ray irradiation of U343 glioma cells and a mouse cancer model.
  • Assessment of DNA damage, cell death, and TP53 activity.

Main Results:

  • Reduced Per2 expression decreased DNA damage and cell death post-irradiation.
  • Per2 was linked to increased TP53 activity and TP53-mediated apoptosis.
  • Per2 appears to be an upstream regulator of TP53.

Conclusions:

  • Per2 functions as a tumor suppressor gene.
  • Per2 inhibits tumor growth by promoting TP53 expression, DNA damage repair, and apoptosis.
Keywords:
TP53U343 cellsX-raygliomaperiod2

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