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Related Experiment Video

Updated: Mar 23, 2026

Comet Assay to Quantify DNA Damage in FLT3 Mutant-expressing 32D Cells after Exposure to Type I and Type II FLT3 Inhibitors

Comet Assay to Quantify DNA Damage in FLT3 Mutant-expressing 32D Cells after Exposure to Type I and Type II FLT3 Inhibitors

Published on: October 17, 2025

The comet assay: Reflections on its development, evolution and applications.

Narendra P Singh¹

¹Department of Bioengineering, Box 355061, University of Washington, Seattle, WA 98195, USA.

Mutation Research. Reviews in Mutation Research

|April 3, 2016

Summary

This summary is machine-generated.

The comet assay, a simple technique, quantifies DNA damage and repair cell-by-cell. This method is crucial for understanding aging and cancer, with growing applications in biological research.

Keywords:

Aging Cancer Comet assay DNA damage and repair Microgel electrophoresis Single cell gel electrophoresis

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Last Updated: Mar 23, 2026

Comet Assay to Quantify DNA Damage in FLT3 Mutant-expressing 32D Cells after Exposure to Type I and Type II FLT3 Inhibitors

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Published on: May 10, 2022

CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells

CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells

Published on: October 18, 2014

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

DNA damage accumulation is linked to aging and cancer.
A sensitive, cell-by-cell method was needed to quantify DNA damage and repair.
The comet assay emerged to meet this need in aging research.

Discussion:

The development of the comet assay involved overcoming obstacles to improve sensitivity and reliability.
The review details the personal journey and inspirations behind the assay's creation.
Standardization, modifications, and applications of the comet assay are discussed.

Key Insights:

The comet assay enables sensitive, single-cell level measurement of DNA damage.
Its application has expanded across diverse cell types and species.
The technique has become a global standard for DNA damage monitoring.

Outlook:

The comet assay's use is increasing with the focus on single-cell biological phenomena.
Clinical relevance is growing, potentially illuminating human aging and prevention.
Future applications may further advance our understanding of DNA damage and repair in health and disease.