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Spatial-scanning hyperspectral imaging probe for bio-imaging applications.

Hoong-Ta Lim¹, Vadakke Matham Murukeshan¹

¹Centre for Optical and Laser Engineering, School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798.

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Summary

This study introduces a novel spatial-scanning hyperspectral imaging probe, enabling endoscopic applications. The developed probe offers advanced bio-imaging capabilities for hard-to-access tissues.

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Area of Science:

Optics and Photonics
Biomedical Imaging
Spectroscopy

Background:

Hyperspectral imaging (HSI) is crucial for detailed sample analysis.
Existing HSI probes primarily use spectral-scanning or snapshot methods.
Spatial-scanning HSI probes have been limited, particularly for endoscopic use.

Purpose of the Study:

To develop and validate a spatial-scanning (pushbroom) hyperspectral imaging probe.
To integrate this probe with an endoscopic imaging system.
To assess its performance for bio-imaging applications.

Main Methods:

Integration of a pushbroom hyperspectral imager with a custom image fiber bundle probe.
Utilizing a gradient-index lens for probe configuration.
Performance validation using standard resolution charts and biological tissue samples.

Main Results:

Successful development of a functional spatial-scanning hyperspectral imaging probe.
Demonstrated capability for endoscopic use via an integrated image fiber bundle.
Validated resolution and performance on test targets and chicken breast tissue.

Conclusions:

The developed spatial-scanning hyperspectral imaging probe expands HSI capabilities into endoscopic and difficult-to-access regions.
This technology holds significant potential for diagnostic bio-imaging.
The probe offers advantages in acquiring multi-spectral data from diverse samples.