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Related Concept Videos

Constant Pressure Calorimetry03:02

Constant Pressure Calorimetry

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Calorimetry is a technique used to measure the amount of heat involved in a chemical or physical process or to measure the heat transferred to or from a substance. The heat is exchanged with a calibrated and insulated device called the calorimeter. Calorimetry experiments are based on the assumption that there is no heat exchange between the insulated calorimeter and the external environment. The well-insulated calorimeters prevent the transfer of heat between the calorimeter and its external...
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Constant Volume Calorimetry02:41

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Calorimeters are useful to determine the heat released or absorbed by a chemical reaction. Coffee cup calorimeters are designed to operate at constant (atmospheric) pressure and are convenient to measure heat flow (or enthalpy change) accompanying processes that occur in solution at constant pressure. A different type of calorimeter that operates at constant volume, colloquially known as a bomb calorimeter, is used to measure the energy produced by reactions that yield large amounts of heat and...
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Calorimetry01:19

Calorimetry

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When objects at different temperatures are placed in contact with each other but isolated from everything else, they attain thermal equilibrium. A container that prevents heat transfer in or out is called a calorimeter, and the use of a calorimeter to make measurements is called calorimetry. Generally, these measurements involve heat or specific heat capacity. The term "calorimetry problem" is used for any problem where the specified objects are thermally isolated from their...
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Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Related Experiment Video

Updated: Mar 23, 2026

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
08:13

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

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High-pressure differential scanning microcalorimeter.

A A Senin1, L N Dzhavadov2, S A Potekhin1

  • 1Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.

The Review of Scientific Instruments
|April 3, 2016
PubMed
Summary

A novel differential scanning microcalorimeter was developed to study biopolymer conformational changes under high pressure. This instrument enables precise measurements of heat capacity, advancing biopolymer research.

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Synthesis and Microdiffraction at Extreme Pressures and Temperatures
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Last Updated: Mar 23, 2026

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Synthesis and Microdiffraction at Extreme Pressures and Temperatures
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Synthesis and Microdiffraction at Extreme Pressures and Temperatures

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Area of Science:

  • Biophysics
  • Biochemistry
  • Physical Chemistry

Background:

  • Understanding biopolymer behavior under extreme conditions is crucial for various biological and industrial applications.
  • Thermotropic conformational transitions influence protein stability and function.
  • High pressure can significantly alter these transitions.

Purpose of the Study:

  • To design and present a novel differential scanning microcalorimeter (DSC) capable of high-pressure measurements.
  • To enable the study of thermotropic conformational transitions in biopolymers at elevated pressures.

Main Methods:

  • Development of a specialized differential scanning microcalorimeter.
  • Operation at pressures up to 3000 atm.
  • Measurement of partial heat capacity of biopolymer solutions as a function of temperature.

Main Results:

  • Successful design and implementation of a high-pressure DSC.
  • Demonstration of the capability to measure biopolymer heat capacity under pressures up to 3000 atm.
  • Discussion of calibration methods and potential applications.

Conclusions:

  • The developed microcalorimeter is a valuable tool for investigating pressure-induced effects on biopolymer structure.
  • This technology opens new avenues for studying protein stability and conformational dynamics under high-pressure conditions.