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Related Experiment Video

Updated: Mar 23, 2026

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

Published on: April 5, 2021

Isolating Lysosomes from Rat Liver.

¹Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York, York YO10 5DD, United Kingdom.

Cold Spring Harbor Protocols

|April 3, 2016

Summary

This summary is machine-generated.

This study details a rapid method for isolating functional lysosomes from rat liver using density-gradient centrifugation. The resulting lysosome-rich fraction is suitable for in vitro assays, preserving cellular integrity.

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Last Updated: Mar 23, 2026

Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

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Published on: April 5, 2021

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Area of Science:

Cell Biology
Biochemistry
Molecular Biology

Background:

Lysosomes are crucial organelles involved in cellular degradation and waste removal.
Maintaining lysosome integrity and function is vital for biochemical and cellular studies.

Purpose of the Study:

To describe a protocol for generating a lysosome-enriched fraction from rat liver.
To ensure isolated lysosomes are functional for in vitro applications.

Main Methods:

Rapid isolation of lysosomes via density-gradient centrifugation.
Utilizing gradient media that preserves lysosome osmolarity and function.

Main Results:

Successful generation of a fraction highly enriched in rat liver lysosomes.
Isolated lysosomes maintained functional integrity for subsequent assays.

Conclusions:

This protocol provides an efficient method for obtaining functional lysosomes.
The technique is valuable for researchers conducting in vitro studies on lysosomal function.