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Isolating Lysosomes from Rat Liver
1Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York, York YO10 5DD, United Kingdom.
Cold Spring Harbor Protocols
|April 3, 2016
Abstract:
This protocol describes the generation of a fraction enriched in lysosomes from rat liver. The lysosomes are rapidly isolated using density-gradient centrifugation with gradient media that retain the osmolarity of the lysosomes such that they are functional and can be used in in vitro assays.
