Analysis of prasugrel active metabolite R-138727 in human plasma: a sensitive, highly selective and fast LC-MS/MS...

Area of Science:

• Analytical Chemistry
• Pharmacology

Background:

• Prasugrel is an antiplatelet medication.
• Accurate quantification of its active metabolite is crucial for clinical studies.

Purpose of the Study:

• To develop and validate a sensitive, rapid, and cost-effective LC-MS/MS method for quantifying the prasugrel active metabolite (R-138727) in human plasma.

Main Methods:

• High-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed.
• Solid phase extraction was used for sample preparation.
• Isocratic elution on a reverse phase C18 column and multiple reaction monitoring (MRM) were utilized for analysis.

Main Results:

• The method demonstrated a linear dynamic range of 0.2-120 ng/mL.
• The lower limit of quantification was 0.2 ng/mL with a 5.0% relative standard deviation.
• Intra- and inter-batch precision and accuracy were within acceptable ranges (3.9-9.6% and 95.2-102.2%, respectively).

Conclusions:

• A validated, simple, and repeatable LC-MS/MS method for prasugrel metabolite quantification in human plasma was established.
• This method is suitable for bioequivalence and pharmacokinetic studies.