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Inhibition of murine melanoma growth by sodium ascorbate
1Department of Chemistry, Rhodes University, Grahamstown, South Africa.
Abstract:
Ascorbic acid has been reported to play a role in treatment and prevention of cancer. This study was carried out to determine the effect of ascorbate on growth of normal LLCMK cells and transformed BL6 cells in cell culture and on the growth of BL6 melanomas in vivo. Ascorbic acid levels were also measured to determine the effect of tumor growth and supplementary ascorbate on cellular ascorbic acid levels. Ascorbate addition at levels of up to 200 micrograms/ml was found to inhibit the in vitro growth of BL6 cells but not of LLCMK cells. Ascorbic acid levels in both cell types were very similar. The presence of tumors was found to reduce liver ascorbic acid levels in mice. Supplementary dietary ascorbate increased liver and tumor ascorbic acid levels and also reduced the growth of BL6 melanomas transplanted in C57 mice. Ascorbate thus appears to play a role in suppression of BL6 melanoma growth.