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Published on: September 28, 2018
TWO-DIMENSIONAL GEL ELECTROPHORESIS ANALYSIS OF BROWN ALGAL PROTEIN EXTRACTS(1)
Loretto Contreras1, Andrés Ritter1, Geraldine Dennett1
1Center for Advanced Studies in Ecology & Biodiversity, Departamento de Ecología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile Center for Advanced Studies in Ecology & Biodiversity, Departamento de Ecología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile CNRS, Université Pierre et Marie Curie, UMR 7139, Station Biologique, Roscoff, FranceCenter for Advanced Studies in Ecology & Biodiversity, Departamento de Ecología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, ChileDepartamento de Biología, Facultad de Química y Biología, Universidad de Santiago, Santiago, Chile High-Throughput Proteomics Platform OUEST-genopole®, UPRES JE 2459, INSERM, U625, Campus de Beaulieu F-35042, FranceDepartamento de Biología, Facultad de Química y Biología, Universidad de Santiago, Santiago, ChileCNRS, Université Pierre et Marie Curie, UMR 7139, Station Biologique, Roscoff, FranceCenter for Advanced Studies in Ecology & Biodiversity, Departamento de Ecología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
Abstract:
High-quality protein extracts are required for proteomic studies, a field that is poorly developed for marine macroalgae. A reliable phenol extraction protocol using Scytosiphon gracilis Kogame and Ectocarpus siliculosus (Dillwyn) Lyngb. (Phaeophyceae) as algal models resulted in high-quality protein extracts. The performance of the new protocol was tested against four methods available for vascular plants and a seaweed. The protocol, which includes an initial step to remove salts from the algal tissues, allowed the use of highly resolving two-dimensional gel electrophoresis (2-DE) protein analyses, providing the opportunity to unravel potentially novel physiological processes unique to this group of marine organisms.
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