Related Experiment Video
Updated: Mar 23, 2026
Author Spotlight: Investigating Immune Cell Dynamics in the Tumor Microenvironment — Challenges and Innovations in Cancer Prognosis
Published on: April 12, 2024
Immunohistochemical detection of proliferating cell nuclear antigen in hepatocellular carcinoma
1Dong Wang, MD, Department of Clinical Pathology, Daping Hospital, Third Military Medical University, Chongqing 630042, China.
Aim:
To investigate the expression levels of proliferating cell nuclear antigen (PCNA) and its correlation with the mitotic count, histological grade, and metastasis of human hepatocellular carcinoma (HCC).
Methods:
PCNA expression was detected immunohistochemically with PC10 monoclonal antibody for 80 cases of HCC. The PCNA labeling index (LI) was assessed by counting the positively-stained nuclei per 500 cells.
Results:
PCNA LI ranged from 1.8% to 91.4% (mean = 33.9%) in cancerous tissues, and was significantly related to the tumor size, histological grade, mitotic count and the metastases found in HCC samples.
Conclusion:
HCC Proliferation activity, as defined by PCNA immunohistochemical analysis, is strongly correlated with tumor invasiveness. These findings hold potential prognostic value for HCC patients.
