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Low-cost, disposable microfluidics device for blood plasma extraction using continuously alternating paramagnetic and
Pilkee Kim1, Eng Hui Ong1, King Ho Holden Li1
1School of Mechanical and Aerospace Engineering, Nanyang Technological University , 50 Nanyang Ave., Singapore 639798.
Biomicrofluidics
|April 5, 2016
Summary
This study introduces a low-cost, disposable microfluidics device for high-purity blood plasma extraction. The device utilizes magnetophoresis to efficiently separate blood cells, improving diagnostic accuracy.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Biophysics
Background:
- Blood plasma analysis is crucial for diagnosing diseases and assessing physiological conditions.
- High purity of blood plasma is essential for accurate diagnostic testing.
- Current plasma separation methods can be costly and complex.
Purpose of the Study:
- To develop a low-cost, disposable microfluidics device for efficient blood plasma extraction.
- To utilize magnetophoretic behavior of blood cells for plasma separation.
- To simplify the device structure for mass production and improve diagnostic accuracy.
Main Methods:
- Design and simulation of a modular microfluidics device with a disposable chip and reusable magnetic field source.
- Application of alternating magnetophoretic capture modes to separate paramagnetic and diamagnetic blood cells.
- Experimental validation of the device for blood plasma extraction from deoxygenated blood.
Main Results:
- Numerical simulations and parametric studies elucidated the blood cell separation mechanism.
- Experimental tests demonstrated successful extraction of pure blood plasma.
- A yield of 21.933% plasma was achieved from a 75 μl sample of 1:10 diluted deoxygenated blood.
Conclusions:
- The proposed microfluidics device offers a cost-effective and efficient solution for blood plasma separation.
- The modular design facilitates simplified manufacturing and potential for widespread adoption in diagnostics.
- This technology holds promise for enhancing the accuracy and accessibility of blood-based diagnostic tests.
