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Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools

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Exploring membrane respiratory chains.

Bruno C Marreiros¹, Filipa Calisto¹, Paulo J Castro¹

¹Instituto de Tecnologia Química e Biológica-António Xavier, Universidade Nova de Lisboa, Av. da República EAN, 2780-157 Oeiras, Portugal.

Biochimica Et Biophysica Acta

|April 5, 2016

Summary

This summary is machine-generated.

This study reveals the vast diversity of membrane respiratory chains across life, detailing the presence of key enzyme complexes involved in energy acquisition and transmembrane potential maintenance. Understanding these systems offers a novel perspective on cellular energetics.

Keywords:

Anaerobe Ion transport Oxygen Quinone Respiration Taxonomic profile

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Area of Science:

Bioenergetics
Microbiology
Biochemistry

Background:

Energy acquisition is fundamental for life, supporting processes beyond ATP synthesis, such as metabolite transport and motility.
Membrane potential, crucial for cellular functions, is established and maintained by membrane-bound respiratory complexes.
Understanding the diversity of these complexes is key to comprehending the range of energetic metabolisms across different life forms.

Purpose of the Study:

To explore the diversity of membrane respiratory chains and enzyme complexes across various phyla.
To create taxonomic profiles of membrane-bound respiratory proteins and complexes by analyzing coding genes in the KEGG database.
To investigate the presence and distribution of quinone reductases, quinol:electron carriers oxidoreductases, terminal electron acceptor reductases, and associated enzymes.

Main Methods:

Systematic analysis of KEGG database for coding genes of membrane-bound respiratory proteins and complexes.
Taxonomic profiling of 26 quinone reductases, 5 quinol:electron carriers oxidoreductases, and 18 terminal electron acceptor reductases.
Inclusion of enzymes involved in ion translocation, ATP synthase, transhydrogenase, and electron carriers like quinones and soluble proteins.

Main Results:

Comprehensive taxonomic profiles of diverse membrane-bound respiratory complexes were generated.
The study identified the presence and distribution of numerous enzyme complexes across different phyla.
A broad perspective on the functional connections between membrane complexes, electron carriers, and energetic metabolism was established.

Conclusions:

The findings provide a novel, integrated view of membrane-bound respiratory complexes and their role in cellular energetics.
This research enhances our understanding of the diversity of energetic metabolisms in living systems.
The study contributes valuable data for future research in bioenergetics and evolutionary microbiology.