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Related Concept Videos

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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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ADP/ATP carrier or AAC protein is the most abundant carrier protein in the inner mitochondrial membrane. It transports large quantities of ADP and ATP, equivalent to the average human body weight, every day. Among other transporters, ACC protein is one of the best-studied members of the mitochondrial carrier protein family. The ADP/ATP carrier protein comprises two transmembrane helices connected to a loop and a single alpha-helix on the matrix side. It switches between two conformational...
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At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
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The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
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Related Experiment Video

Updated: Mar 23, 2026

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
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Doa1 is a MAD adaptor for Cdc48.

Ting Zhang1, Yihong Ye2

  • 1Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.

The Journal of Cell Biology
|April 6, 2016
PubMed
Summary
This summary is machine-generated.

The p97/Cdc48 ATPase complex dislocates polypeptides from the mitochondrial outer membrane. Doa1 acts as a crucial adaptor, recruiting substrates to Cdc48 for processing in degradation pathways.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Mitochondrial Biology

Background:

  • The p97/Cdc48-Ufd1-Npl4 ATPase complex is vital for protein dislocation from the mitochondrial outer membrane.
  • This process is essential for mitochondria-associated degradation and Parkin-mediated mitophagy, key cellular quality control mechanisms.

Purpose of the Study:

  • To identify novel components involved in the substrate recruitment to the p97/Cdc48 complex.
  • To elucidate the role of these components in protein dislocation and subsequent degradation pathways.

Main Methods:

  • Biochemical assays to study protein-protein interactions.
  • Cellular imaging techniques to visualize protein localization and mitochondrial dynamics.
  • Genetic manipulation to assess the function of identified proteins in degradation pathways.

Main Results:

  • Doa1 was identified as a pivotal adaptor protein.
  • Doa1 directly recruits specific substrates to the Cdc48 complex.
  • This recruitment facilitates the dislocation of polypeptides from the mitochondrial outer membrane.

Conclusions:

  • Doa1 plays a critical role as an adaptor in the p97/Cdc48-mediated dislocation pathway.
  • The identification of Doa1 provides new insights into the regulation of mitochondria-associated degradation and mitophagy.