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The fingerprint, a false-positive fern test
J G Lodeiro1, K A Hsieh, J H Byers
1Department of Obstetrics and Gynecology, State University of New York Health Science Center, Syracuse.
Abstract:
Microscope slides were prepared to confirm the presence of ferning from a 32-week gestation with possible premature rupture of the membranes. Although no amniotic fluid was seen in the vagina, an atypical ferning pattern was produced and was again reproducible with fingerprints on the glass slide. Guidelines to recognize and avoid this source of error are outlined.
Insights
Atypical ferning patterns on microscope slides can mimic amniotic fluid, even without vaginal fluid. This study highlights common errors in ferning tests and provides guidelines to prevent misdiagnosis of premature rupture of membranes.
Area of Science:
- Obstetrics and Gynecology
- Clinical Pathology
Background:
- Premature rupture of membranes (PROM) is a critical obstetric complication.
- Ferning tests are commonly used to diagnose PROM by detecting amniotic fluid crystals.
Observation:
- An atypical ferning pattern was observed on microscope slides.
- This pattern was reproducible using fingerprints on a glass slide.
- No amniotic fluid was present in the vaginal samples.
Findings:
- The observed atypical ferning was not indicative of amniotic fluid.
- Fingerprint contaminants can create false-positive ferning test results.
- This highlights a potential source of error in PROM diagnosis.
Implications:
- Misinterpretation of ferning tests can lead to incorrect PROM diagnosis.
- Clinicians must be aware of potential contaminants causing false positives.
- Implementing guidelines can improve diagnostic accuracy for premature rupture of membranes.