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[Expression of FAS-AS1 in breast cancer and its effect on the host gene sFas]
1Department of Head & Neck, and Breast Surgery, Xinxiang Central Hospital, Xinxiang, Henan 453000, China.
Objective:
To investigate the expression level of antisense transcript of FAS (FAS-antisense 1, FAS-AS1) in breast cancer, and its effect on the host gene soluble Fas receptor (sFas).
Methods:
The expression of FAS-AS1 and sFas gene was detected in 20 cases of breast cancer tumors and breast cancer cells and their normal counterparts by using real-time quantitative polymerase chain reaction(RT-qPCR). The effects of FAS-AS1 on the expression of sFas gene in breast cancer cells were analyzed by overexpressing FAS-AS1 in breast cancer cells.
Results:
FAS-AS1 expression in breast cancer tissue and cell lines was significantly lower than that of adjacent breast tissue (P=0.000) and normal breast cells (P<0.01). However, sFasexpression in the breast cancer tissue and cell lines was significantly higher than that of adjacent breast tissue (P=0.001) and normal breast cells (P<0.01). The expression of FAS-AS1 was negatively correlated with the expression of sFas(r=-0.751, P=0.020). FAS-AS1 expression was observed to be closely correlated with tumor size (r(2)=0.351, P=0.025) and lymph node metastasis (r(2)=0.265, P=0.043). Expression of sFas and cell proliferation was significantly reduced in FAS-AS1-overexpressing MCF-7 cells (P<0.05).
Conclusion:
FAS-AS1 is distinctly downexpressed in breast cancer, and it has certain effects on the expression of host gene sFas and breast cancer development.
Insights
Antisense transcript of FAS (FAS-AS1) is downregulated in breast cancer, inversely correlating with soluble Fas receptor (sFas) expression. FAS-AS1 overexpression reduced sFas and cell proliferation, suggesting a role in breast cancer development.
Area of Science:
- Molecular biology
- Cancer research
- Genetics
Background:
- Breast cancer is a significant global health concern.
- The role of non-coding RNAs, such as antisense transcripts, in cancer is an emerging area of research.
- Soluble Fas receptor (sFas) is involved in apoptosis and immune regulation, and its dysregulation is implicated in various cancers.
Purpose of the Study:
- To investigate the expression level of FAS-antisense 1 (FAS-AS1) in breast cancer.
- To determine the relationship between FAS-AS1 expression and the expression of its host gene, soluble Fas receptor (sFas).
- To explore the functional impact of FAS-AS1 on breast cancer cells.
Main Methods:
- Real-time quantitative polymerase chain reaction (RT-qPCR) was used to measure FAS-AS1 and sFas gene expression in breast cancer tissues and cell lines.
- FAS-AS1 was overexpressed in breast cancer cells to analyze its effects on sFas expression.
- Correlation analyses were performed to assess the relationship between FAS-AS1, sFas, tumor size, and lymph node metastasis.
Main Results:
- FAS-AS1 expression was significantly lower in breast cancer tissues and cells compared to normal counterparts.
- sFas expression was significantly higher in breast cancer tissues and cells.
- FAS-AS1 expression showed a negative correlation with sFas expression and was associated with larger tumor size and lymph node metastasis.
- Overexpression of FAS-AS1 in MCF-7 cells led to reduced sFas expression and cell proliferation.
Conclusions:
- FAS-AS1 is significantly downregulated in breast cancer.
- FAS-AS1 influences the expression of its host gene, sFas.
- FAS-AS1 may play a role in breast cancer development and progression.
