Related Experiment Video

Updated: Mar 23, 2026

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.5K

SETD7 Controls Intestinal Regeneration and Tumorigenesis by Regulating Wnt/β-Catenin and Hippo/YAP Signaling

Menno J Oudhoff1, Mitchell J S Braam1, Spencer A Freeman2

  • 1The Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.

Developmental Cell
|April 6, 2016
PubMed

Insights

The enzyme SETD7 is crucial for intestinal cancer growth and regeneration by linking the Wnt/β-catenin and Hippo/Yes-Associated Protein (YAP) pathways. SETD7 methylation of YAP promotes Wnt signaling, driving tumor development.

Area of Science:

  • Molecular biology
  • Cancer research
  • Gastroenterology

Background:

  • Intestinal tumorigenesis involves mutations in signaling pathways controlling cell growth and survival.
  • Wnt/β-catenin pathway mutations are common in intestinal cancers.
  • The Hippo/Yes-Associated Protein (YAP) pathway is an emerging regulator of intestinal tumorigenesis and regeneration.

Purpose of the Study:

  • To investigate the role of lysine methyltransferase SETD7 in Wnt-driven intestinal tumorigenesis and regeneration.
  • To elucidate the mechanism by which SETD7 links the Wnt/β-catenin and Hippo/YAP pathways.

Main Methods:

  • Investigated SETD7's requirement in Wnt-driven intestinal tumorigenesis and regeneration models.
  • Analyzed the interaction of SETD7 with YAP, AXIN1, and β-catenin.
  • Assessed the effect of SETD7-dependent YAP methylation on β-catenin nuclear accumulation.

Main Results:

  • SETD7 is essential for Wnt-driven intestinal tumorigenesis and regeneration.
  • SETD7 forms a complex with YAP, AXIN1, and β-catenin.
  • SETD7-mediated YAP methylation promotes Wnt-induced nuclear accumulation of β-catenin.

Conclusions:

  • SETD7 acts as a methyltransferase linking the Wnt/β-catenin and Hippo/YAP pathways.
  • This methyltransferase-dependent mechanism is critical for intestinal regeneration and tumorigenesis.
  • Targeting SETD7 may offer a novel therapeutic strategy for intestinal cancers.

More Related Videos

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

20.8K
Culturing and Manipulation of O9-1 Neural Crest Cells08:32

Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

11.2K

Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.6K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.8K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.9K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

2.6K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
10.3K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

1.8K