DNA immunization as a technology platform for monoclonal antibody induction

Area of Science:

• Immunology
• Molecular Biology
• Infectious Diseases

Background:

• Emerging infectious diseases pose a significant global health threat.
• Monoclonal antibodies (mAbs) are crucial tools for diagnostics and therapeutics.
• Traditional protein-based immunization has limitations in generating specific mAbs.

Purpose of the Study:

• To provide an updated summary of DNA immunization for producing high-quality monoclonal antibodies (mAbs).
• To analyze the literature and present findings on DNA immunization for mAbs against emerging infectious diseases.
• To discuss critical factors influencing the success of DNA immunization.

Main Methods:

• Comprehensive literature analysis of DNA immunization for mAb production.
• Review of studies focusing on mAbs against emerging infectious diseases.
• Inclusion of the authors' own research and experience.

Main Results:

• DNA immunization is efficient for testing immunogen designs without protein purification.
• It is effective for generating mAbs against conformation-sensitive targets.
• DNA immunization facilitates the production of highly functional mAbs.

Conclusions:

• DNA immunization is a powerful approach for developing mAbs against diverse pathogens, especially for emerging infectious diseases.
• Optimizing immunogen design, delivery, immunization schedules, and immune modulators is critical for success.
• This review offers a detailed guide to leveraging DNA immunization for advanced mAb generation.