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Updated: Mar 23, 2026

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events
Published on: August 27, 2019
Dynamic control of cell adhesion on a stiffness-tunable substrate for analyzing the mechanobiology of collective cell
Masao Kamimura1, Michiko Sugawara, Shota Yamamoto
1WPI Research Center Initiative, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba 305-0044, Japan. NAKANISHI.Jun@nims.go.jp.
Abstract:
A method was developed for photocontrolling cell adhesion on a gel substrate with defined mechanical properties. Precise patterning of geometrically controlled cell clusters and their migration induction became possible by spatiotemporally controlled photo-irradiation of the substrate. The clusters exhibited unique collective motion that depended on substrate stiffness and cluster geometry.
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