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Digital Microfluidics for Automated Proteomic Processing
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A digital microfluidic interface between solid-phase microextraction and liquid chromatography-mass spectrometry.

Kihwan Choi1, Ezel Boyacı2, Jihye Kim3

  • 1Department of Chemistry, University of Toronto, 80 St George St., Toronto, ON M5S 3H6, Canada; Donnelly Centre for Cellular and Biomolecular Research, 160 College St., Toronto, ON M5S 3E1, Canada; Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science, Yuseong-gu, Daejeon 34113, Republic of Korea.

Journal of Chromatography. A
|April 7, 2016
PubMed
Summary
This summary is machine-generated.

We developed a novel method combining solid-phase microextraction (SPME) with digital microfluidics (DMF) and HPLC-MS. This technique efficiently extracts and concentrates analytes from complex samples for sensitive analysis.

Keywords:
Digital microfluidicsHPLC-MSHormoneSPMESolid-phase microextractionSteroidUrine

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Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Chromatography

Background:

  • Solid-phase microextraction (SPME) is a widely used technique for sample preparation.
  • High-performance liquid chromatography-mass spectrometry (HPLC-MS) is a powerful analytical tool for complex mixtures.
  • Integrating SPME with HPLC-MS can improve sensitivity and reduce analysis time.

Purpose of the Study:

  • To develop a novel method coupling SPME with HPLC-MS using digital microfluidics (DMF).
  • To enable automated, multiplexed processing of SPME fibers.
  • To achieve pre-concentration of analytes for enhanced sensitivity.

Main Methods:

  • SPME fibers were used to extract analytes from sample solutions.
  • Analytes were desorbed into solvent droplets within a DMF device.
  • Automated droplet manipulation in DMF facilitated multiplexed fiber processing.
  • The integrated SPME-DMF-HPLC-MS system was applied to quantify steroid hormones in urine.

Main Results:

  • The SPME-DMF-HPLC-MS method allowed for straightforward insertion of SPME fibers.
  • The open geometry of DMF simplified the interface requirements.
  • Low volumes in DMF enabled efficient pre-concentration of analytes.
  • The method successfully quantified picogram per milliliter levels of free steroid hormones in urine.

Conclusions:

  • The developed SPME-DMF-HPLC-MS method offers a convenient and efficient approach for sample preparation and analysis.
  • The system facilitates multiplexed processing and pre-concentration of analytes.
  • This technique holds promise for various applications requiring cleanup and pre-concentration coupled with high-performance analytical methods.