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Related Concept Videos

Embryonic Connective Tissues01:20

Embryonic Connective Tissues

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During early development, the embryo forms two types of connective tissues— the mesenchyme and mucoid connective tissue.
The mesenchyme is the first connective tissue that emerges in the developing embryo. It consists of loosely arranged multipotent mesenchymal cells and reticular fibers in the extracellular matrix. This loose arrangement allows easy migration of cells, which is essential for germ layer positioning, patterning, and organ morphogenesis during embryonic development.
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Related Experiment Video

Updated: Mar 23, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
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Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

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Stem cell-derived cell-sheets for connective tissue engineering.

Puay Yong Neo1,2, Thomas Kok Hiong Teh1,2, Alex Sheng Ru Tay1

  • 1a Department of Biomedical Engineering, Faculty of Engineering , National University of Singapore , Singapore.

Connective Tissue Research
|April 7, 2016
PubMed
Summary
This summary is machine-generated.

Cell-sheet technology harvests intact cell layers using temperature-responsive surfaces for tissue engineering. This review explores its connective tissue applications and future potential, including stem cell-derived sheets.

Keywords:
Cell-sheetcell-sheet engineeringconnective tissuestem cells derivedtissue engineering

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Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Biomaterials Science

Background:

  • Cell-sheet technology enables harvesting of contiguous cell layers with extracellular matrix (ECM) and cell-cell junctions intact.
  • Thermoresponsive polymer-grafted surfaces facilitate cell-sheet detachment via controlled surface hydrophobicity changes induced by temperature shifts.

Purpose of the Study:

  • To review the development and applications of cell-sheet technology in tissue engineering.
  • To comprehensively discuss its use in various connective tissues and with stem cells.
  • To highlight recent advances and future prospects.

Main Methods:

  • Review of existing literature on cell-sheet technology and its applications.
  • Detailed discussion of thermoresponsive polymer surfaces and detachment mechanisms.
  • Focus on stem cell-derived cell-sheets and associated challenges.

Main Results:

  • Cell-sheet technology is a versatile tool for regenerating myocardial, vascular, cartilage, bone, tendon/ligament, and periodontal tissues.
  • Stem cell-derived cell-sheets, particularly from mesenchymal stem cells, show significant promise.
  • Challenges in stem cell-sheet applications and recent technological advancements are identified.

Conclusions:

  • Cell-sheet technology is a critical tool in tissue engineering with broad connective tissue applications.
  • The use of stem cell-derived cell-sheets offers significant regenerative potential.
  • Ongoing advancements in cell-sheet formation, detachment, and manipulation will shape future clinical applications.