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Updated: Mar 23, 2026

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
Published on: December 16, 2021
[Micrographic surgery of lentigo maligna]
Renate Ruth van den Bos1, Marlies Wakkee, Senada Koljenovil
1Erasmus Medisch Centrum, Rotterdam.
Abstract:
Lentigo maligna is an in situ melanoma which usually affects sun-damaged skin on the head and neck. In patients with lentigo maligna, the clinically visible demarcation often correlates poorly with the histopathologically confirmed demarcation. The standard treatment for lentigo maligna is excision with at least 5 mm margins. The key disadvantage of regular excision is the lack of histological margin control, resulting in non-radical excisions and a higher risk of recurrence. Micrographic surgery is indicated for patients with lentigo maligna because this technique is tissue-sparing and it offers absolute certainty concerning the presence of clear histological margins.

