Simple and Versatile 3D Printed Microfluidics Using Fused Filament Fabrication

Area of Science:

• Biotechnology
• Materials Science
• Engineering

Background:

• Microfluidics fabrication traditionally requires specialized skills and equipment, hindering wider scientific adoption.
• Limitations in transparency, fidelity, and pressure tolerance have previously challenged microfluidic device development.

Purpose of the Study:

• To present a simplified fabrication method for microfluidic devices using 3D printing.
• To demonstrate the overcoming of traditional limitations in 3D printed microfluidic devices.
• To showcase the utility of these devices in biological applications and ease of prototyping.

Main Methods:

• Utilized an inexpensive, accessible 3D printer and commercially available materials for device fabrication.
• Developed microfluidic devices with integrated ports and a modular, Lego®-like system.
• Performed cell viability assays using encapsulated dental pulp stem cells.

Main Results:

• Achieved high transparency and fidelity in 3D printed microfluidic devices.
• Demonstrated device operation at pressures exceeding 2000 kPa, resolving leakage issues.
• Confirmed high cell viability (>90%) post-encapsulation and sufficient transparency for single-cell imaging.

Conclusions:

• 3D printing provides an accessible and effective method for fabricating high-performance microfluidic devices.
• These novel devices overcome previous limitations, facilitating broader use in research and development.
• The modular system enhances accessibility for novices, promoting rapid prototyping and microfluidic system construction.