Turning a Negative into a Positive: Conversion of a Homodimer into a Heterodimer Using Negative State Repertoires
James A Davey1, Roberto A Chica1
1Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
Structure (London, England : 1993)
|April 7, 2016
Abstract:
In this issue of Structure, Leaver-Fay et al. (2016) engineer bispecific antibodies using multistate computational protein design with negative state repertoires. In combination with additional mutations selected rationally, they produced antibodies that assembled as heterodimers with up to 93% purity.
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