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Effect of atmospheric fine particles on epidermal growth factor receptor mRNA expression in mouse skin tissue
X Han1,2, W L Liang3, Y Zhang1
1Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Abstract:
We investigated the effect of atmospheric fine particles on epidermal growth factor receptor (Egfr) mRNA expression in mouse skin tissue and explored the effect of atmospheric fine particles on skin aging. Forty female BALB/c mice were randomly divided into four groups (each comprising 10 mice) as follows: a saline control group and low-, medium-, and high-dose atmospheric fine particle groups (1.6, 8.0, and 40.0 mg/kg, respectively) (fine particles were defined as those with a diameter of £2.5 mm, i.e., PM2.5). Each dose group was exposed to intratracheal instillation for 3 days. Twenty-four hours after the last exposure, real-time quantitative polymerase chain reaction was used to detect the expression of Egfr mRNA in the skin tissue of each mouse. The expression levels of Egfr mRNA in the medium- and high-dose PM2.5 groups were significantly higher (P < 0.05) than that in the control group, and were positively correlated with the dose. Medium and high concentrations of PM2.5 can induce the expression of Egfr mRNA and promote skin aging.
Insights
Atmospheric fine particles (PM2.5) increase epidermal growth factor receptor (Egfr) mRNA in mouse skin. Exposure to PM2.5 can induce Egfr expression and promote skin aging.
Area of Science:
- Environmental Science
- Dermatology
- Molecular Biology
Background:
- Atmospheric fine particles (PM2.5) are a global environmental concern.
- PM2.5 exposure is linked to various health issues, including skin aging.
- The molecular mechanisms underlying PM2.5-induced skin aging require further investigation.
Purpose of the Study:
- To investigate the effect of PM2.5 on epidermal growth factor receptor (Egfr) mRNA expression in mouse skin.
- To explore the relationship between PM2.5 exposure and skin aging.
Main Methods:
- Forty female BALB/c mice were divided into four groups: saline control, low-, medium-, and high-dose PM2.5.
- Mice received intratracheal instillation of PM2.5 for 3 days.
- Egfr mRNA expression in skin tissue was quantified using real-time quantitative polymerase chain reaction.
Main Results:
- Medium and high doses of PM2.5 significantly increased Egfr mRNA expression compared to the control group (P < 0.05).
- Egfr mRNA expression levels were positively correlated with PM2.5 dose.
- These findings suggest PM2.5 can induce Egfr mRNA expression.
Conclusions:
- Medium and high concentrations of PM2.5 can induce Egfr mRNA expression in mouse skin.
- PM2.5 exposure may promote skin aging through the induction of Egfr.
- Further research is warranted to elucidate the precise mechanisms and long-term effects.
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