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Related Experiment Video

Updated: Mar 23, 2026

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
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Development and characterization of Rift Valley fever virus-like particles.

Y T Li1,2,3, C L Wang1,2, X X Zheng2

  • 1College of Animal Science and Technology, Shihezi University, Shihezi, China.

Genetics and Molecular Research : GMR
|April 7, 2016
PubMed
Summary

Researchers developed a new method to produce Rift Valley fever virus (RVF) virus-like particles (VLPs). This breakthrough paves the way for developing effective RVF vaccines for both animals and humans.

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Area of Science:

  • Virology
  • Vaccinology
  • Biotechnology

Background:

  • Rift Valley fever (RVF) is a zoonotic disease caused by the RVF virus (RVFV), posing significant threats to animal and human health.
  • The disease is endemic in Africa and the Arabian Peninsula, causing considerable economic losses.
  • There is a critical need for novel, effective vaccines against RVFV, as none are currently available for human use.

Purpose of the Study:

  • To develop an efficient method for producing Rift Valley fever virus virus-like particles (VLPs).
  • To lay the groundwork for the development of VLP-based vaccines against RVFV.

Main Methods:

  • Insertion of RVFV N and G protein genes into the pFastBacDual baculovirus expression vector.
  • Transfection of the recombinant vector into Sf9 insect cells to generate recombinant baculovirus (rBac-N-G).
  • Production of RVFV VLPs by infecting Sf9 cells with rBac-N-G, followed by characterization using immunofluorescence, western blot, sucrose density gradient centrifugation, and transmission electron microscopy.

Main Results:

  • Successful generation of RVFV VLPs using the described baculovirus expression system.
  • Confirmation of RVFV protein presence within the VLP preparations via antibody-based assays.
  • Characterization of VLP morphology, which was consistent with native RVFV virions.

Conclusions:

  • A novel and efficient technique for producing RVFV VLPs has been established.
  • The developed VLP production method provides a strong foundation for future VLP-based RVFV vaccine development.