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Updated: Mar 23, 2026

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes
Published on: August 31, 2022
Genetic characterization of a densovirus isolated from great tit (Parus major) in China
Wen-Tao Yang1, Shao-Hua Shi1, Yan-Long Jiang1
1College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal probiotics, Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China.
Abstract:
During a study of ornithophilous viruses in China, a new densovirus (DNV) was isolated from the lung tissue of Parus major (PmDNV-JL). The complete genome of PmDNV-JL was cloned and sequenced. Five open reading frames (ORFs) were identified in the 5166nt sequence, on the basis of deduced amino acids. It was further shown that this virus caused cytopathic effects (CPE) in Feline kidney cells. The NS1 gene sequence of PmDNV-JL shares 70-99% nucleotide sequence identity with isolates of the Blattella germanica densovirus (BgDNV) and BgDNV-like virus. Phylogenetic analysis indicated that the predicted amino acid sequences of capsid (VP) and non-structural domain (NS1) of PmDNV-JL clustered with the BgDNV and were similar to BgDNV-HB within the genus Densovirus.

