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Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid
Published on: May 25, 2022
Viscerotropic velogenic Newcastle disease virus replication in feathers of infected chickens
Dong-Hun Lee1, Jung-Hoon Kwon1, Jin-Yong Noh1
1Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
Abstract:
Newcastle disease viruses (NDVs) cause systemic diseases in chickens with high mortality. However, little is known about persistence of NDVs in contaminated tissues from infected birds. In this study, we examined viral replication in the feather pulp of chickens inoculated with viscerotropic velogenic NDV (vvNDV) genotype VII. Reverse transcription real-time PCR and immunohistochemistry were used to investigate viral persistence in the samples. vvNDV was detected in the oropharynx and cloaca and viral antigens were detected in the feathers, suggesting that feathers act as sources of viral transmission.
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