DNA OF A SEVEN-CHROMOSOME STRAIN OF ASTREPHOMENE GUBERNACULIFERA(1) (2)

Methods have been presented for isolation, partial purification, and characterization of DNA from a 7-chromosome strain of Astrephomene gubernaculilera. Sheath material surrounding the individual cells was removed enzymatically and cells were lysed with hot Na lanryl sulfate. DNA was isolated and purified by a modification of Marmufs procedure. Melting experiments and buoyant-density values indicated that native DNA with a G-C content of 61-62% had been prepared. A pentose phosphorus containing compound was present in stoichiometric amounts.