Related Experiment Video
Updated: Mar 23, 2026
A β-glucuronidase GUS Based Cell Death Assay
Published on: May 6, 2011
Determination of β-glucuronidase in human colorectal carcinoma cell lines
1Shu Feng, Jin-Dan Song, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001, Liaonin Province, China.
Aim:
To study the relationship between β-glucuronidase and the invasiveness of human colorectal carcinoma cell lines.
Methods:
Six colorectal carcinoma cell lines, including three well-differentiated (CX1, CCL187, and CCL229) and three poorly differentiated ones (CCL227, CCL228, and Clone A), were analyzed by Fischman's method to determine the concentration of β-glucuronidase in the medium.
Results:
Low levels of β-glucuronidase (activity range: 1.29 to 1.96 μg/106 cells·h) were associated with poor invasiveness. This finding was in contrast to the elevated levels of the enzyme (2.46-3.37 μg/106·h) detected in the medium derived from the more aggressively invasive cells (CCL 227, CCL 228, Clone A, and CCL 229).
Conclusion:
Highly invasive colorectal carcinoma cells secreted higher levels of β-glucuronidase than the poorly invasive cells. Determination of secreted β-glucuronidase might represent a useful in vitro measurement tool to assess the invasiveness of colorectal carcinoma.
More Related Videos
09:28Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis
Published on: May 12, 2019
10:02Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
Published on: June 2, 2023